Is it possible to run the RTLS Studio in VMware?

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Yes, it is. Please install OS Ubuntu 14.04 Server Edition 64-bit (for RTLS Studio 1.6.x and lower) or OS Ubuntu 18.04 Server Edition 64-bit for RTLS Studio 2.x and higher. To install the RTLS Studio, you can use the install script which is already prepared in our Partner Portal in section Products -> RTLS Studio. See also an Install Guide.